GC-MS profiling and phytochemical quantification in methanolic extract of Bidens tripartita

Eboh Abraham Sisein 1, *, Azibanasamesa DC Owaba 2 and Robert Owabhel Faith 1

1 Department of Biochemistry, Faculty of Basic Medical Science, Niger Delta University, Bayelsa State, Nigeria.
2 Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, Niger Delta University, Wilberforce Island Bayelsa State, Nigeria.
 
Research Article
Global Journal of Research in Chemistry and Pharmacy, 2022, 01(01), 001–006.
Article DOI: 10.58175/gjrcp.2022.1.1.0021
Publication history: 
Received on 20 August 2022; revised on 24 November 2022; accepted on 27 November 2022
 
Abstract: 
Bidens tripartita (Asteraceae) is a weed like plant commonly known as Burmarigold. It is found in water logged and wet regions. The leaves of Bidens tripartita were harvested, dried and grounded. The powdered leaves were extracted with methanol, the extract was processed into a paste and stored in the refrigerator. Standard quantitative spectrophotometric methods were applied to quantify total phenol, flavonoid and alkaloid. Gas chromatography mass spectrometry (GC-MS) evaluation of methanolic leaf extract of Bidens tripartita was according to standard procedures. The phytochemical results showed total phenol 83.35±1.17 mgGAE/g, total flavonoid 10.12±0.05 mgQE/g, total alkaloid 4.5±0.73 %. The GC-MS also reveals many Medicinal compounds like Caryophyllene, Spathulenol, N-butyl-benzenesulfonamide, Methyl palmitoleate, 9,12-octadecadienoic acid methyl ester, Phytol, Gamma tocopherol, Vitamin E, Campesterol, Stigmasterol. Conclusively Bidenstripartita contains a lot of Medicinal phytochemicals that can be harnessed through extensive extraction for the development of a drug.
 
Keywords: 
Phytochemicals; Bidens tripartita; GC-MS; Phenol; Flavonoid; Vitamin E
 
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