Establishment of efficient protocol for proliferation and clonal production of banana through micro propagation

Amar Meghwar, Shahla Karim Baloch *, Muharam Ali, Allah Jurio Khaskheli, Muhammad Rafique Rind and Sadaruddin Chachar

Department of Biotechnology, Faculty of Crop Production, Sindh Agriculture University, Tandojam-70060, Pakistan.
 
 
Research Article
Global Journal of Research in Science and Technology, 2022, 01(01), 001–009.
Article DOI: 10.58175/gjrst.2022.1.1.0011
Publication history: 
Received on 11 August 2022; revised on 18 September 2022; accepted on 21 September 2022
 
Abstract: 
Different concentrations of nutrient media were used, amongst them; the explants cultured on MS media supplementing with IAA 2.00 mg l-1 + BAP 2.00 mg l-1 + NAA 1.0 mg l-1 acquired least time (19.55 days) to regenerate. observed at beneath the centralization of MS+ IAA 1.00 mg l-1 + BAP 2.00 mg l-1 and recorded the least days taken at benchmark (26.67 days) underneath the MS+ IAA 2.5 mg, MS+ IAA 1.00 mg l-1 + BAP 2.00 mg l-1 and the least days taken at pattern had been accounted for (26.67 days). Results with diverse obsessions uncovered that the most noteworthy shoot span of bott l-1 (15.90 cm) become archived under concentrate MS+ IAA 2.5 mg l-1 + BAP 3.5 mg l-1 + NAA 2.5 mg l- 1, followed by way of (14.05 cm) under listen MS+ IAA 1.00 mg l-1 + BAP 2.00 mg l-1 whilst bottle1 became accounted for underneath listen MS+ IAA 2.5 mg l-1 + BAP 3.5 mg l-1 + NAA 2.5 mg l-1 decrease shoot period (8.47 cm). Results from one-of-a-kind centers uncovered that extra bottle1 leaves had been received (7.33) beneath the MS+ IAA 2.00 mg l- 1 + BAP2.00 mg l-1 + NAA 1.0 mg l-1 centralization, trailed by (6.89) under the MS+ IAA 1.00 mg l-1 + BAP 2.00 mg l-1 accumulating, while decrease bottle1 leaves were enrolled (3.89) under the MS+ IAA 2.5 mg l-1 + BAP 3.5 mg l-1 + NAA 2.5 mg l-1 obsession. The maximum extreme number of root bottles1 changed into reached (24.11) underneath the characterization of MS1⁄2 + IBA 2.00 mg 1 + IAA 3.00 mg l-1 + sucrose 75 g l-1, went earlier than (21.55) via (21.55) MS1⁄2 + IBA 2.00 mg l-1 + IAA 3.00 mg l-1 + sucrose 70 g l-1 whilst the least number of root bottles1 (14.77) was discovered underneath the obsession of MS1⁄2 + IBA 2.00 mg l-1 + IAA 3.00 mg l-1 + sucrose 70 g l-1.
 
Keywords: 
Banana; Mass-Clonal Production; Proliferation; Micro propagation
 
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