Establishment of efficient protocol for proliferation and clonal production of banana through micro propagation

Different concentrations of nutrient media were used. Among them, the explants cultured on MS media supplemented with IAA 2.00 mg L^-1 + BAP 2.00 mg L^-1 + NAA 1.0 mg L^-1 required the least time (19.55 days) to regenerate. The next response was observed at the concentration of MS + IAA 1.00 mg L^-1 + BAP 2.00 mg L^-1 and recorded the benchmark days to regenerate (26.67 days) under MS + IAA 2.5 mg L^-1 and MS + IAA 1.00 mg L^-1 + BAP 2.00 mg L^-1.

Results with diverse concentrations revealed that the highest shoot length (15.90 cm) was archived under MS + IAA 2.5 mg L^-1 + BAP 3.5 mg L^-1 + NAA 2.5 mg L^-1, followed by 14.05 cm under MS + IAA 1.00 mg L^-1 + BAP 2.00 mg L^-1, while the lowest shoot length (8.47 cm) was recorded under MS + IAA 2.5 mg L^-1 + BAP 3.5 mg L^-1 + NAA 2.5 mg L^-1.

Results from different concentrations revealed that more leaves were obtained (7.33) under the MS + IAA 2.00 mg L^-1 + BAP 2.00 mg L^-1 + NAA 1.0 mg L^-1 concentration, followed by 6.89 under the MS + IAA 1.00 mg L^-1 + BAP 2.00 mg L^-1 group, while fewer leaves were registered (3.89) under the MS + IAA 2.5 mg L^-1 + BAP 3.5 mg L^-1 + NAA 2.5 mg L^-1 concentration.

The maximum number of roots was reached (24.11) under the combination of MS ½ + IBA 2.00 mg L^-1 + IAA 3.00 mg L^-1 + sucrose 75 g L^-1, followed by 21.55 under MS ½ + IBA 2.00 mg L^-1 + IAA 3.00 mg L^-1 + sucrose 70 g L^-1, while the least number of roots (14.77) was observed under MS ½ + IBA 2.00 mg L^-1 + IAA 3.00 mg L^-1 + sucrose 70 g L^-1.